Volume 7

January-March 2015

Anticerous activity of L-asparaginases enzyme produced from Aspergillus niger by solid state fermentation

U Salmanul Faris, O M Fasalu Rahiman, Shejina M, Mohthash Musambil

Abstract:
Today a number of research works are carried out in production of enzymes from an isolated organism, and evaluating its effect on animal studies. L-Asparaginase is now known to be a potent antineoplastic agent in animals and has given complete remission in some human leukemia’s. Extensive clinical trials of this enzyme, however, were not possible in the past because of inadequate production. Strain improvement of organisms is one alternative method that can be implemented to tackle this problem. Strain improvement can enhance the production, and increase the activity of enzymes produced. L-asparaginases are found to be responsible for catalyzing the deamination of Asparagines to yield Aspartic acid and an ammonium ion, resulting in depletion of free circulatory Asparagines in plasma. Its use in therapeutics is found to be remarkable, especially for those specific cases where blood cells become cancerous, such as in acute lymphoblastic leukemia. The commercial availability of L-asparaginase has revolutionized the molecular therapy of acute lymphocytic leukemias and melanosarcoma. Therefore, highly purified and effective Asparaginase is of great importance for today’s practical interests. The present study deals with successful isolation and purification of L-asparaginase enzyme from Aspergillus niger by solid state fermentation. L-asparaginase produced was purified and characterized. The anticerous activity of the enzyme was studied on human cancer cell line by MTT Assay method and the results showed clear positive results

Keywords: Anticancerous Enzyme, L-asparaginase, MTT Assay, Aspergillus niger, Solid State Fermentation.