Abstract:
The present investigation was evaluated the potential antimicrobial activity of patchouli oil (procured from fresh and dried patchouli leaf extracts, cultivated in Indian acidic soil zone). Extraction of patchouli oil was carried out by hydrodistillation method using Clevenger apparatus. The content of patchouli alcohol was estimated by Gas chromatography (GC) method. Microbiocides of patchouli oil was evaluated against several microorganisms viz. Bacillus substilis, Staphylococcus aureus, Streptococcus pyogenes, Enterobacter aerogenes, Pseudomonus aeruginosa, Escherichia coli, Klebsiella pneumoniae and Serratia marcescens by agar diffusion technique. The Minimum Inhibition Concentration (MIC) of the patchouli oil was appointed by the dilution method in the tube and the results revealed the concentration dependent (p<0.001) potential antimicrobial activity of both the oils by determined with zone of inhibition against standard ampicillin. At the dose of 300 mcg/ml patchouli oil gave maximum zone of inhibition against Staphylococcus (14.53±0.37**) followed by 12.15 ± 0.35** against Streptococcus from the second year of harvested. Such variation may be due to the effects of rich organic carbon content in acidic soil that increased the quality of oil content in patchouli leaves (collected from second year harvested fresh leaves). It proved patchouli is a strong potential antimicrobial plant. ><0.001) potential antimicrobial activity of both the oils by determined with zone of inhibition against standard ampicillin. At the dose of 300 mcg/ml patchouli oil gave maximum zone of inhibition against Staphylococcus (14.53±0.37**) followed by 12.15 ± 0.35** against Streptococcus from the second year of harvested. Such variation may be due to the effects of rich organic carbon content in acidic soil that increased the quality of oil content in patchouli leaves (collected from second year harvested fresh leaves). It proved patchouli is a strong potential antimicrobial plant.

Keywords: Antimicrobial Study, Acidic Soil, Agar Diffusion Technique, Hydrodistillation, Patchouli Oil, Patchouli Alcohol.