Abstract:
Accumulation of asiaticoside (an important bioactive compound) was determined in parent, first subcultured & second subcultured calluses obtained from leaf explants of Centella asiatica (L.) Urban. In first subcultured callus the accumulation of asiaticoside was 0.125 mg/g dry mass, using leaf explants in presence of napthelene acetic acid (NAA 1mg/l) and benzyl amino purine (BAP 2mg/l), was the highest among all the callus cultures studied, but this accumulation of asiaticoside was lower than that obtained from whole plant material (0.179 mg/g dry mass).

Keywords: Centella asiatica, Callus Culture, Subculture, Asiaticoside.