Volume 12

October-December 2020​

Improved antibacterial activity of ‎honey, propolis and beeswax-‎sodium carboxymethyl cellulose ‎composite hydrogel

Mohamed Hamdi, Baghdad Khiati, Moussa Ahmed

Abstract:

Honey, propolis, and beeswax are natural products that have been used in alternative medicine and traditional practices. This study aimed to develop an antibiotic hydrogel containing propolis, honey, and beeswax and evaluation of its antibacterial potentials. Fourier transform infrared (FTIR) analysis was employed to characterise the NaCMC-H-P-BW hydrogel. Besides, the antibacterial activity of composite hydrogel was determined by using agar well diffusion assay against Staphylococcus aureus and Escherichia coli, and the zone of inhibition was measured in terms of millimeters. A topical cream containing 1% silver sulfadiazine was used as a positive control and distilled water will be used as the negative control. Fourier transform infrared spectroscopy (FTIR) analysis suggested the formation of strong intermolecular interactions as hydrogen, oxygen, and carbon attractions between polymer and propolis, honey and beeswax. The antibacterial properties showed the inhibition activity against Staphylococcus aureus (23.10±4.60 mm) and E. coli (22.95±4.76 mm).  Moreover, the inhibition activity observed for the positive control (silver sulfadiazine) was comparatively less compared to NaCMC-H-P-BW hydrogel Staphylococcus aureus (8.25±0.95) and Escherichia coli  (9.30±1.53). These studies suggest that the topical formulation could be used as an antibacterial topical apitherapeutic product.

Keywords: NaCMC, Honey, Propolis, Beeswax, Antibacterial Activity.