Volume 10

July-September 2018

Preparation and characterization of ‎marigolds (Cosmos caudatus L.) ‎Leaf extract-loaded nanoemulsion: ‎Physicochemical properties and in-‎vitro release activities of ‎nanoemulsion system using virgin ‎coconut oil (VCO)‎

Rahmi Annisa, ‎Roihatul Muti’ah, ‎Siti Maimunah, ‎Begum Fauziyah, ‎Zahratunnahdhati ‎Li’ibaadatillaah

Abstract: 
Marigolds (Cosmos caudatus L.) leaves widely consumed as a vegetable and empirically been used as a medicine for various diseases. In this study, nanoemulsion was prepared using leaf extract of C. caudatus with a concentration of 5%, 10%, 15% and using Virgin Coconut Oil (VCO) as an oil phase. The purpose of this study is to know the characteristics of the formulation and to determine the effect of extract concentration on the release rate of the active compound of quercetin of C. caudatus leaf extract on the preparation. C. caudatus extract was obtained by the ultrasonic method with 96% methanol solvent. Nanoemulsion extracts were made by a low energy method. All formulations were evaluated for the preparation characteristics which included organoleptic test, pH test, particle size, emulsion type, entrapment efficiency, and stability, and quercetin release test of C. caudatus leaf extract using Franz cell diffusion method with cellophane membrane. The result of the characteristic evaluation of the research shows that formulation 1, 2, and 3 give a good result that is in accordance with the standard specification of each specified. The quercetin release flux of each nanoemulsion preparation formulation was 13,374 ± 0.216 μg / cm2 / min, 9,617 ± 0.404 μg / cm2 / min and 8,635 ± 0.021 μg / cm2 / min respectively. This result shows that the higher of extract concentration, the longer the release time.

Keywords: Marigolds (Cosmos caudatus L.) leaf, Quercetin, Nanoemulsion, Extract Concentration, Release.