Volume 3

April-June 2011

RP-HPLC method for simultaneous estimation of levamisole, mebendazole and albendazole in pharmaceutical products

Kullai Reddy Ulavapalli, J Sriramulu, Useni Reddy Mallu, Viswanath Reddy Pyreddy, Varaprasad Bobbarala

Abstract:
Single and reproducible RP-HPLC method has been developed for the simultaneous estimation of Levamisole, Mebendazole and Albendazole in pharmaceutical products. Chromatographic separation was achieved by using Inertsil ODS-3V C18, 250 x 4.6 mm, 5μm column, mobile phase composed of sol-A: Potassium dihydrogen phosphate (1.0 gram in 1000 ml of HPLC Water ) buffer and sol-B: Acetonitrile with gradient elution (0-5min- sol-A: 80-80; 5-7min- sol-A: 80-60; 7- 10min- sol-A: 60-30; 10-15min- sol-A: 30-80 and 15-20min- sol-A: 80-80). Flow rate was 1.00 ml per min and measured the absorbance at 210nm. The retention time of Levamisole, Mebendazole and Albendazole are 4.8min, 12.8min and 14.1min, respectively. The linearity of the method was evaluated from 5µg per mL to 100µg per mL for each ingredient and the correlation coefficient result was observed for each ingredient was not less than 0.999. The developed method has wide applicable in the quantification of Levamisole, Mebendazole and Albendazole in pharmaceutical dosage forms.

Keywords: Levamisole, Mebendazole, Albendazole, RP-HPLC Method.