Acute toxicity and anti-ulcer activity of mixture leaves-flowers extracts of Cytisus triflorus L.
Soraya Madoui, Kamel Mokhnache, Hanane Khither, Seoussen Kada, Noureddine Charef
The extract of leaves-flowers mixture of Cytisus triflorus L was selected for their anti-ulcer effect and oral acute toxicity investigation. The acute toxicity method described in European guideline 425, is a method of administering a specified oral dose of 2 g/kg of the extract, to exclude possible toxicity due to extract, a histological study of the liver and kidneys was performed. The results showed the absence of liver and renal toxicity following the administration of the 2 g / kg dose of the crude leaves and flower mixture extract of Cytisus triflorus L. Concerning gastric antiulcer activity, it is necessary to check the protective action of the crude extract at doses 200 mg/kg and 400 mg/kg, against the ulcer caused in animals by administration of an ulcerogenic agent such as ethanol. The evaluation of the gastroprotective effect of the extract was carried out by macroscopic and microscopic analysis which will look at the observable external and internal lesions, as well as an estimate of the percentage of ulceration. The results show a very significant reduction in the areas injured in the Ranitidine treated groups and the crude extract at doses of 200 or 400 mg / kg, with percentages of 0.75 ± 0.16%, 2.71 ± 0.13. % and 1.49 ± 0.14% respectively. The gastroprotective effect of the crude extract at 400 mg/kg is confirmed by the restoration of the normal architecture of the rats’ stomachs. These results confirm the validity of the use of this plant in traditional medicine and offer hope for the development of effective anti-ulcer therapy without side effects.
Keywords: Cytisus Triflorus, Mixture, Anti-Ulcer, Acute Toxicity.