Abstract:
The aim of this study is to evaluate the antioxidant and anti-inflammatory activities of aqueous (AqE) and ethanolic (EE) extracts of Sinapis alba L.. The antioxidant activity was carried out by free radical scavenging method (DPPH) and bleaching of β-carotene, the anti-inflammatory was evaluated in vitro according to the test of inhibition protein (ovalbumin) denaturation and in vivo by the ear edema test induced by xylene (topical application). According to obtained results, total polyphenols content in ethanolic extract was 133.625±4.309μgGAE/mgE, while in aqueous extract was 87.533±7.416μg GAE/mgE. Ethanolic extract was found to be richer in flavonoids (22.199±0.763μgQE/mgE) in comparison with aqueous extract (14.068±1.308μgQE/mgE). In the DPPH assay, ethanolic extract showed the higher scavenging capacity (IC₅₀ = 0.097±0.013mg/ml) followed by aqueous extract with IC₅₀ of 0.162±0.01mg/ml. Whereas, ethanolic extract showed the best inhibitory capacity of the coupled oxidation of linoleic acid/ β-carotene (71.024±12.9%). The anti-inflammatory activity in vitro was 66% and 72% in aqueous and ethanolic extracts respectively compared to diclofenac 62%, the anti-inflammatory activity in vivo showed that after 2 hours of the treatment by aqueous extract produced a significant inhibition 87%.

Keywords: Sinapis alba L., Polyphenols, Antioxidant Activity, Anti-inflammatory Activity.