Abstract:
The present study was carried out to determine the chemical composition and to evaluate anticancer and antimicrobial activities of essential oil extracted from Citrus volkameriana leaves. GC-MS analysis of the essential oil obtained from C. volkameriana leaves revealed that the major compounds were limonene (21.26%), sabinene (14.14%), linalool (9.64%), Citronellal (7.08%) and Terpinene-4-ol (6.23%). The growth inhibitory effect of the essential oil were tested in the 3-cell lines panel consisting of TK10 (renal), UACC62 (melanoma) and MCF7 (breast) cancer cells using Sulforhodamine B (SRB) assay. Antimicrobial activity was tested against four gram positive bacteria, four gram negative bacteria strains, gram stain resistant microbe Mycobacterium tuberculosis and against four fungal yeasts. The oil showed a significant activity on MCF7 Breast cancer cell line and antimicrobial activity especially on Micrococcus luteus B-287 with MIC (31.25 µg/ml).

Keywords: Citrus volkameriana, Leaves Essential Oil, Limonene, Anticancer Activity, Antimicrobial Activity.