Abstract: 
Liposomes are versatile, self-assembled vesicles with applications spanning pharmaceuticals, cosmetics, and biotechnology. This review provides a comprehensive examination of liposomes, focusing on their classification, preparation methods, and compositional aspects. We first delve into the various classification schemes of liposomes, including unilamellar and multilamellar structures, and their subcategories based on size and charge. Next, we explore the diverse methodologies for liposome preparation, ranging from traditional techniques such as the thin-film hydration method to advanced methods like microfluidic mixing and solvent injection. Additionally, we discuss the impact of lipid composition, including the roles of phospholipids, cholesterol, and other additives, on the physicochemical properties and functionality of liposomes. By synthesizing current knowledge and highlighting recent advancements, this review aims to provide a holistic understanding of liposome technology and its implications for future research and application.

Keywords: Liposomes, Liposome Classification, Liposome Preparation Methods, Liposome Composition, Phospholipids, Cholesterol.