Abstract: 
Peganum harmala L. belongs to the family Zygophyllaceae, and it is one of the most widely used plants in Algerian folk medicine in the treatment of jaundice and relieving joint pain and rheumatism. The aim of this study is to contribute to the evaluation of some biological activities of alkaloids extract of P. harmala roots, which are grown in Sidi El-Raghis region – Oum El Bouaghi country. Using the classical method of extraction of β-carboline alkaloids, the yield of alkaloids in the roots was estimated at 2.34%. Harmine  and  harmaline  were  the  components  identified  in  total alkaloids  roots extract  by  using  high  performance  liquid  chromatography  method. The analysis of antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP)  methods showed that the crude alkaloids extract of P. harmala had a significant activity in capturing free radicals (IC₅₀= 46.89±0.31 μg/mL), (EC₅₀=0.12±0.05 μg/mL), respectively. The results of the anti-inflammatory activity showed for the alkaloid compounds, there were significant differences compared to the control compound represented by diclofenac, the best value was recorded with the harmine compound (EC₅₀= 22.71± 0.52μg/mL). We conclude from this research, that harmala alkaloids have anti-oxidant and anti-inflammatory properties, this study is a good indication in the future development of plant-based drugs against anti-inflammatory and antioxidant activity.

Keywords: Anti-inflammatory Activity, Antioxidant Activity, Crude Alkaloids, Harmaline, Harmine, Peganum harmala.