Abstract:
Ayurvedic eye drops preparation contains aqueous extracts of different herbs. Ethnobotanical survey shows that plants used in Ayurvedic eye drops formulation are rich source of tannin and tannin like compounds. Tannins are responsible for antimicrobial and antioxidant properties of Ayurvedic eye drops. The present study was designed with the aim to determine the tannin content in 3 different brands of Ayurvedic eye drops, by colorimetric method using Folin-denis reagent. The tannin content of all the three brands was found to be A-825.23, B-656.00 and C-558.49 µg/ml. The results obtained are reproducible with coefficient of variation less than 0.99 %. The present approach can be used as one of the parameters for the standardization of Ayurvedic eye drop preparations.

Keywords: Ayurvedic Eye Drops, Tannins, Folin-denis Reagent, Spectrophotometer.