Volume 3

July-September 2011

Surface modified implant - release of antibiotic in the presence of aerobic microorganisms

Ganesh Krishnamoorthy, Sridharan M, Umamaheswari Krishnan, Swaminathan Sethuraman

Aerobic microorganisms are responsible for majority of infections. By means of formation of biofilms, resistance against various drug delivery systems are been reported. Most of the aerobic microorganisms are catalase and superoxide dismutase positive. The main objective of this study is to create antibiotic loaded implants, which releases antibiotics during the presence of aerobic microorganisms, thereby preventing the formation of biofilms and improving the treatment efficacy. Stainless steel plates were coated by dip coating method. The solution was prepared by using Polyhydroxybutyrate-co-hydroxyvalerate in Dichloromethane and followed by addition of ofloxacin. By spray coating technique, sodium formate was coated over the polymer film. Parameters such as in vitro drug release, zone of inhibition were studied. Coated plates with uniform thickness of 9µm were obtained. In all bacterial strains, Staphylococcus aureus, Clostridium sporogens, Pseudomonas aeruginosa, the zone of inhibition was noticed with a inhibited zone area of 64mm for S. aureus and 19mm for C. sporogenes. The in vitro drug release study showed that the total encapsulated drug was released over a period of 6 hours. Antibiotic coated plate’s offers a new perspective for treating implant related infections and also overcoming formation of biofilms. The study can be applied for creating biosensors to detect microorganisms.

Keywords: Aerobic Organisms, Catalase, Infections, Superoxide Dismutase, Ofloxacin.